ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the therapeutic effectiveness of fusion tumor vaccine in tongue cancer treatment.</p><p><b>METHODS</b>Human macrophages fused with human tongue carcinoma cell line Tca8113 cell. The fusion cells were selected by magnetic cell sorting (MACS) and cultured. The biological properties of fusion cells and anti-tumor immune response in vitro induced by fusions were observed.</p><p><b>RESULTS</b>In contrast to Tca8113, the fused cells grew significantly slow in vitro. The expression of MHC I, II antigen of the fusion cells which was detected by flow cytometry (FCM) was higher than that of Tca8113. The fused cells significantly increased the proliferation of mixed lymphocyte and induced their cytotoxicity on parental Tca8113.</p><p><b>CONCLUSIONS</b>The fusion tumor vaccine of macrophages and OSCC cells increase in vitro immunogenicity significantly. This indicates that fusion tumor vaccine could be a new method of anti-tumor immunotherapy, which has important potentials for effective individualized human OSCC vaccine.</p>
Subject(s)
Animals , Humans , Rats , Cancer Vaccines , Allergy and Immunology , Carcinoma, Squamous Cell , Allergy and Immunology , Cell Fusion , Cell Line, Tumor , Histocompatibility Antigens , Allergy and Immunology , In Vitro Techniques , Macrophages , Allergy and Immunology , Tongue Neoplasms , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVE</b>To present an established human chordoma cell line for chordoma research.</p><p><b>METHODS</b>The specimens pathologically identified as chordoma were cultured, using primary tissue culture in vitro. The surviving cells were analyzed by morphology, histochemical stain, cell cycling analysis, karyotype analysis, electron microscopic observation, heterotransplantation and study of invasive capacity in vitro.</p><p><b>RESULTS</b>The newly established cell line CM-319 has been maintained in continual cultures for over 100 generations in two years. Its morphological observation, histochemical staining properties, electron microscopic observation and heterotransplantation showed the common characteristics of chordoma. The doubling time of cells was about 33 hours. Cell cycle analysis showed: G(1) 55.6%, G(2) 21.9% and S 22.5%, G(2)/G(1) = 1.90. Chromosome analysis showed a hypotriploid feature and the success rate of heterotransplantation was 100%. It is capable of invasion in vitro.</p><p><b>CONCLUSION</b>CM-319, as a cell line derived from human chordoma cells, may serve for further studies of chordoma.</p>